It is a little known fact that trypsin cleaves at lysine residues with lesser efficiency than at arginine residues. Trypsin 2500 umg is a serine protease found in the digestive system useful in removing cells from the surface of a culture. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. Trypsin nb premium grade, ms approved trypsin nb sequencing grade, modified high purity absence of chymotryptic activity 0 400 800 1200 1600 2000 02 468 10 12 14 time min absorption mau promega ko14806 purity of trypsin sequencing grade, modified and promega trypsin gold, mass spectrometry grade in reversed phase hplc. Gold biotechnology is about putting our quality products into capable hands to leads to discoveries that impact the entire world. Jul 24, 20 promegasigma bobinetrypzean promega trypsin is a good choice to start as this product has been around for a long time.
Trypsin gold, mass spectrometry grade technical bulletin pdf 398 kb english. At goldbio, we believe that putting our quality products in capable hands leads to discoveries that impact the entire world. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Trypsin ingel digestion in gel reduction and alkylation. Efficient digestion with trypsin lysc mix in the presence of trypsin inhibiting agents. Incubate overnight at 37 c speedvac, add 200 ul water and speedvac again. Use of trypsin lysc resulted in fewer missed cleavages and a. It consists of a single chain polypeptide of 223 amino acid residues. For improved ingel digestion and protein solubilization. Reconstitute promega trypsin gold or sequencing grade porcine trypsin in acetic acid as advised by the manufacturer. Trypsin gold, mass spectrometry grade trypsin digestion. The treated trypsin is then purified by affinity chromatography and lyophilized.
Sequencing grade modified trypsin, promega supplier. A 500ng sample of carbonic anhydrase ii was separated by gel electrophoresis and digested with 500ng of trypsin gold, mass spectrometry grade, overnight at 37c. Addition of tp standard to serva trypsin nb products allows for additional internal calibration. Promega sequencing grade modified trypsin, lyophilized.
V5280 trypsin goldmass spec grade, 100ug components. Trypsinlys c mix, mass spec grade, is a mixture of trypsin gold, mass spectrometry grade, and rlysc, mass spec grade. Dilute trypin in 50 mm ammonium bicarbonate 10 ngul, add enough to cover the gel pieces 1015 ul. Our success relies on our commitment to the success of those whom we supply.
Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. The sequencing grade of modified trypsin has been further improved by. Promega trypsin has been modified by reductive methylation, rendering it extremely resistant to autolytic digestion. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsinlike activity. Instructions for use of products e2920, e2940, e2980. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.
The specificity of the purified trypsin is further improved by tpck treatment, which inactivates chymotrypsin. Chymotrypsin is a highlypurified serine endopeptidase derived from bovine pancreas that preferentially hydrolyzes at the carboxyl side of aromatic amino acids. Proteasomeglo trypsinlike assay from promega description the proteasomeglo cellbased assays are homogeneous, luminescent assays that individually measure the chymotrypsinlike, trypsinlike and caspaselike protease activities associated with the proteasome in a purified enzymebased format. Tp standard has to be added to the trypsin solution in a ratio of 1.
Promega trypsin gold, mass spectrometry grade 100ug. Comparison of alternative approaches to trypsin protein. Goldbios competent cells deliver up to 10x higher transformation efficiency than competitors. Our success relies on our commitment to the success. Trypsin gold, mass spectrometry grade, is manufactured to provide maximum specificity.
Modified trypsin is maximally active in the range of ph 79 and is reversibly inactivated at ph mass spectrometry grade, has extremely high specific activity and is extremely resistant to autolytic digestion. Pepsin can be used alone or with other proteases for protein analysis by mass spectrometry and other applications. Modified trypsin is maximally active in the range of ph 79 and is reversibly inactivated at ph trypsin gold, mass spectrometry grade, overnight at 37c. Pepsin preferentially cleaves at the cterminus of phenylalanine, leucine, tyrosine and tryptophan.
Modified trypsin is maximally active in the range of ph 79 and is reversibly inactivated at ph promega. Elastase has a unique capability of digesting elastin. A copy of the spectrogram generated with carbonic anhydrase ii and any lot of trypsin gold, mass spectrometry grade, may be obtained from promega technical services at 18003569526. Cleavage may also be observed, but at a lower rate, at leu and met. Elastase is a serine protease that preferentially cleaves at the cterminus of alanine, valine, serine, glycine, leucine or isoleucine. Upgrade your cloning projects with the most efficient chemically competent and electrocompetent cells on the market. Improved reproducibility of igg rituximab digestion when using the trypsin lysc mix. All through february, these extremely efficient competent cells are on sale.
Offer does not apply to orders placed through promegaexpress freezers. Nanopure water was prepared with use of milliq water puri. Trypsin gold, mass spectrometry grade, has extremely high specific activity and is extremely resistant to autolytic digestion. Trypsin from porcine pancreas proteomics grade, bioreagent, dimethylated catalog number t6567 storage temperature 28 c technical bulletin ec 3. Trypsin, proteomics grade t6567 technical bulletin. V5280 trypsin gold mass spec grade, 100ug components. Certificate of analysis sequencing grade modified trypsin. Trypsin gold, mass spectrometry grade, was from promega madison, wi. Promega corporation 2800 woods hollow road madison,wi 53711 u. Using tp standard and additional internal calibration, mass accuracy and therewith mascot scores. Trypsin nb premium grade, ms approved trypsin nb sequencing. Trypsin digestion is also an important step in the preparation of peptide fragments for protein analysis using mass spectroscopy ms due to its high specificity and reproducibility 15. It is resistant to mild denaturing conditions such as 0. The trypsinlys c mix is designed to improve digestion of proteins or protein mixtures in solution.
In the dlr assay, the activities of firefly photinus pyralis and renilla renilla reniformis, also known as sea pansy luciferases are measured sequentially from a. Sequencing grade trypsin has been manufactured to provide maximum specificity. With promega enzyme a mascot scoreof 156 was identified. The dualluciferase reporter dlr assay system provides an efficient means of performing dualreporter assays. In the dlr assay, the activities of firefly photinus pyralis and renilla renilla reniformis, also known as sea pansy luciferases are measured sequentially from a single sample. Tools for improved protein mass spec sample preparation by. Serva trypsin nb premium grade was compared to promega trypsin gold fig. Immobilized trypsin provides a fast, convenient method for digesting a range of concentrations of purified protein or complex protein mixtures.
Promega sequencing grade modified trypsin, lyophilized sequencing grade trypsin has been manufactured to provide maximum specificity. Protocol for sample preparation using a smart digest trypsin kit smart digest samples were diluted to 2 mgml in water. Promega sequencing grade modified trypsin, lyophilized 5 x. Trypsin gold, mass spectrometry grade 100g v5280 trypsinlysc mix, mass spec grade 20g v5071 100g v5072 100g 5. Mass spectrometry compatible surfactant for optimized in. Modified trypsin is maximally active in the range of ph 79 and is reversibly inactivated at ph trypsin is then purified by affinity chromatography and lyophilized to yield trypsin gold, mass spectrometry grade. A significantly higher of 165 was achieved for serva trypsin. To produce trypsin gold, mass spectrometry grade, lysine residues in the porcine trypsin are modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant. Trypsin was added to samples at a trypsinprotein ratio of 1.
Maximum digest specificity with extreme resistance to autolytic digestion. Proteasomeglo trypsinlike assay g8632 from promega. Each lot of qualitytested trypsin gold, mass spectrometry grade, is qualified for use with ingel digestion and mass spectrometric analysis. Trypsin is a member of the serine protease s1 family. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Digested peptides are separated from the trypsin as they flow through the spin column into the collection tube. Then, 150 l smart trypsin buffer was added to smart digest vials.
894 1136 1378 69 1255 644 524 309 442 515 1432 842 52 871 805 825 1540 1236 1453 559 1372 371 155 834 970 905 684 1084 111 816 966 1073 228 1383